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  • Towards biochemical filters with a sigmoidal response to pH changes: buffered biocatalytic signal transduction.

Towards biochemical filters with a sigmoidal response to pH changes: buffered biocatalytic signal transduction.

Physical chemistry chemical physics : PCCP (2011-01-25)
Marcos Pita, Vladimir Privman, Mary A Arugula, Dmitriy Melnikov, Vera Bocharova, Evgeny Katz
ABSTRACT

We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid--the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element" for increasing the complexity of biochemical information processing systems. Evaluation of an optimal regime for quality filtering is accomplished in the framework of a kinetic rate-equation model.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl butyrate, 99%
Supelco
Ethyl butyrate, analytical standard