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  • Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection.

Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection.

Revista da Sociedade Brasileira de Medicina Tropical (2020-03-19)
Ana Lia Pradella Puglia, Murilo de Freitas Peigo, Fernando Russo Costa Bomfim, Ronaldo Luis Thomasini
ABSTRACT

Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of the host and can reactivate after the primary infection, leading to lytic replication in immunosuppressed patients. This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) to identify HHV-7 serum antibodies and compare its performance with that of an indirect immunofluorescence assay (IFA). Serum samples (n=102) were tested by IgG-IFA and by ELISA. IFA and ELISA showed IgG-positive results in 77 and 73 samples, respectively. Qualitative concordance of 96% was demonstrated between the two techniques. ELISA may be useful to diagnose HHV-7 infection.