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  • In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing.

In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing.

Bulletin of experimental biology and medicine (2018-03-28)
S V Nikulin, M P Raigorodskaya, A A Poloznikov, G S Zakharova, U Schumacher, D Wicklein, C Stürken, K Riecken, K A Fomicheva, B Ya Alekseev, M Yu Shkurnikov
PMID29582205
ABSTRACT

IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.