TBST for Western Blotting

Tris-buffered saline with 0.1% Tween^® 20 detergent (TBST) is an effective wash buffer for many immunoassays. To make 1 L of TBST wash buffer, add 100 mL of 10X TBS and 1 mL Tween^® 20 detergent to 900 mL of water.

This calculator enables the accurate preparation of a 1X TBST working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.

1X Solution concentrations:

• Tris: 20 mM
• NaCl: 150 mM
• Tween^® 20 detergent: 0.1% (w/v)

TBST Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Phosphate-Buffered Saline (PBS) Recipe Calculator
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions