MABF2212

Anti-SARS-CoV Nsp8 Antibody, clone 5A10

• Synonym(s): Nonstructural protein NS8, ORF8
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5A10, monoclonal
• mol wt: calculated mol wt 14 kDa; observed mol wt ~22 kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: N/A
• UniProt accession no.: A0A6B9VTP3
• storage temp.: 2-8°C
• Gene Information: vaccinia virus ... orf8(43740577)

Description

Specificity

Clone 5A10 is a mouse monoclonal antibody that detects Nonstructural protein NS8 of SARS-CoV-2.

Immunogen

GST-tagged recombinant Nonstructural protein NS8 from SARS-CoV-2.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 Nsp8 protein.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected recombinant SARS-CoV-2 Nsp8 protein.

Tested Applications

Western Blotting Analysis: A representative lot detected Nsp8 in Western Blotting applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

Immunofluorescence Analysis: A representative lot detected NSP8 in Immunofluorescence applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Nonstructural protein NS8 (UniProt: A0A6B9VTP3; also known as ORF8 protein) is encoded by the orf8 (also known as NS8) gene (Gene ID: 43740577) in Sars-CoV-2. Coronaviruses (CoV) represent a diverse family of positive-sense RNA viruses capable of causing respiratory and enteric disease in human and animal hosts. Amongst these the SARS-CoV-2 is the most prominent virus that causes severe respiratory syndrome in human. The nonstructural proteins (nsps) of coronavirus assemble to form the replication transcription complex (RTC) responsible for viral RNA synthesis. In SARS-CoV-2 non-structural protein Nsp8 is a critical component of the RNA replicase and features both well folded secondary structure and disordered segments. It is part of the RNA-dependent RNA polymerase (RdRp) complex and forms a heterotetramer consisting of one molecule of Nsp7, two copies of Nsp8 and one of Nsp12. Nsp8 an Nsp7 are reported to adopt a hollow cylinder-like structure and serve as co-factors for the catalytic Nsp12 that play a role in the stabilization of Nsp12 regions involved in RNA binding and are essential for a highly active Nsp12 polymerase complex. (Ref.: Trevino, MA., et al. (2023). Nucleic Acids Res. 51(18); 10041-10048; Couroble, VV., et al. (2021). J. Am. Soc. Mass Spectrom. 32(7); 1618-1630; Kumar, P., et al. (2007). Virology. 366; 293-303).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable