CA1026
Anti-Wilm′s Tumor Protein Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-WT-1
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... WT1(7490)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm′s tumor protein.
Recognizes the ~47-55 kDa Wilm′s tumor protein in Wilm′s tumor tissue.
This Anti-Wilm′s Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm′s Tumor Protein.
Immunogen
Human
a synthetic peptide corresponding to amino acids from the C-terminus of human Wilm′s tumor protein
Application
Immunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.6.
Reconstitution
Following initial use, aliquot and freeze (-20°C) for long-term storage.
Analysis Note
Positive Control
Wilm′s tumor tissue
Wilm′s tumor tissue
Other Notes
Huff, V. 1998. Am. J. Med. Genet.79, 260.
Staining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noortje A M Bax et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 239(8), 2307-2317 (2010-07-27)
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Timo H Lüdtke et al.
American journal of physiology. Lung cellular and molecular physiology, 316(5), L767-L783 (2019-02-01)
The mesothelial lining of the lung, the visceral pleura, and of the heart, the epicardium, derive from a common multipotent precursor tissue, the mesothelium of the embryonic thoracic cavity that also contributes to organ-specific mesenchymal cell types. Insight into mesothelial
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