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12-309

Sigma-Aldrich

HeLa Nuclear Extract

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25 μG
$182.00

$182.00

Estimated to ship onMay 26, 2025

A recombinant, preservative-free antibody is available for your target. Try ZMS1029
Request a Bulk Order
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25 μG
$182.00

About This Item

UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.41

$182.00

Estimated to ship onMay 26, 2025

A recombinant, preservative-free antibody is available for your target. Try ZMS1029
Request a Bulk Order

biological source

human

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

concentration

2.0 mg/mL

technique(s)

western blot: suitable

shipped in

dry ice

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species reactivity

rabbit, monkey, sheep, mouse, Xenopus, human, pig, canine, rat

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

mouse, rat, human

clone

C464.6, monoclonal

clone

polyclonal

clone

polyclonal

clone

C464.6, recombinant monoclonal

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

Gene Information

human ... ATP1A3(478)

Gene Information

human ... ATP1A2(477)
rat ... Atp1A2(24212)

Gene Information

rat ... Atp1A1(24211)

Gene Information

rat ... Atp1A1(24211)

UniProt accession no.

P05023

UniProt accession no.

P06686

UniProt accession no.

P06687

UniProt accession no.

P05023

General description

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

Cell Line Description

HeLa

Quality

Routinely evaluated as a positive control for many of Upstates antibodies.

Physical form

RIPA sample buffer containing inhibitors. Frozen solution.

Storage and Stability

Stable for 6 months at -70°C from date of shipment.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    J D Dignam et al.
    Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
    We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major
    Tabetha Sundin et al.
    Biochimie, 94(12), 2639-2648 (2012-08-21)
    Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the

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