HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)
Materials
analytical column
Product No.
Description
Pricing
Zenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 15 cm × 7.8 mm, 3 μm particle size, stainless steelstandard
Product No.
Description
Pricing
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)Ribonuclease A from bovine pancreas
for molecular biology, ≥70 Kunitz units/mg protein, lyophilizedCONDITIONS
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm, 300 Å particles (Z777028)
mobile phase
150 mM potassium phosphate, pH 6.8
flow rate
as indicated on figure
column temp.
ambient
detector
UV, 280 nm
injection
10 μL
sample
each 1 mg/mL except uracil, 2.5 mg/mL
Description
Legal Information
Zenix is a registered trademark of Sepax Technologies