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  • Ritonavir-induced hepatotoxicity and ultrastructural changes of hepatocytes.

Ritonavir-induced hepatotoxicity and ultrastructural changes of hepatocytes.

Ultrastructural pathology (2014-08-01)
Chang-Chun Kuang, Ying Wang, Peng-Chao Hu, Fang-Fang Gao, Lang Bu, Xian-Mei Wen, Qing-Ming Xiang, Hui Song, Qun Li, Lei Wei, Ke Li
ABSTRACT

To investigate the effect of ritonavir on hepatocyte proliferation, we detected the change of cleaved caspase-3 expression level in the hepatocytes. Furthermore, the morphological and ultrastructural changes of hepatocytes derived from RTV-treated mice have been observed. The results showed that ritonavir can evidently inhibit hepatocyte proliferation and increase cleaved caspase-3 expression level. Under the electron microscope, chromatin margination, mitochondrial cristae disappearance, karyopyknosis and cytoplasmic vacuolization can be observed in the hepatocytes of mice treated with ritonavir. In conclusion, the mechanism of ritonavir's hepatotoxicity is that it induces apoptosis of hepatocytes via the caspase-cascade system.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ritonavir, ≥98% (HPLC)
Ritonavir, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)