Skip to Content
MilliporeSigma
  • Local hypersensitivity reaction in transgenic mice with squamous epithelial IL-5 overexpression provides a novel model of eosinophilic oesophagitis.

Local hypersensitivity reaction in transgenic mice with squamous epithelial IL-5 overexpression provides a novel model of eosinophilic oesophagitis.

Gut (2012-11-20)
Joanne C Masterson, Eóin N McNamee, Lindsay Hosford, Kelley E Capocelli, Joseph Ruybal, Sophie A Fillon, Alfred D Doyle, Holger K Eltzschig, Anil K Rustgi, Cheryl A Protheroe, Nancy A Lee, James J Lee, Glenn T Furuta
ABSTRACT

Eosinophilic oesophagitis (EoE) is a chronic inflammatory condition of the oesophagus with limited treatment options. No previous transgenic model has specifically targeted the oesophageal mucosa to induce oesophageal eosinophilia. We developed a mouse model that closely resembles EoE by utilising oxazolone haptenation in mice with transgenic overexpression of an eosinophil poietic and survival factor (interleukin (IL)-5) in resident squamous oesophageal epithelia. Overexpression of IL-5 in the healthy oesophagus was achieved in transgenic mice (L2-IL5) using the squamous epithelial promoter Epstein-Barr virus ED-L2. Oxazolone-challenged L2-IL5 mice developed dose-dependent pan-oesophageal eosinophilia, including eosinophil microabscess formation and degranulation as well as basal cell hyperplasia. Moreover, oesophagi expressed increased IL-13 and the eosinophil agonist chemokine eotaxin-1. Treatment of these mice with corticosteroids significantly reduced eosinophilia and epithelial inflammation. L2-IL5 mice provide a novel experimental model that can potentially be used in preclinical testing of EoE-related therapeutics and mechanistic studies identifying pathogenetic features associated with mucosal eosinophilia.

MATERIALS
Product Number
Brand
Product Description

Supelco
4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one, analytical standard, for drug analysis
Pricing and availability is not currently available.
Sigma-Aldrich
4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one, purified by recrystallization
Pricing and availability is not currently available.