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  • Identification and multimodal characterization of a specialized epithelial cell type associated with Crohn's disease.

Identification and multimodal characterization of a specialized epithelial cell type associated with Crohn's disease.

Nature communications (2024-08-22)
Jia Li, Alan J Simmons, Caroline V Hawkins, Sophie Chiron, Marisol A Ramirez-Solano, Naila Tasneem, Harsimran Kaur, Yanwen Xu, Frank Revetta, Paige N Vega, Shunxing Bao, Can Cui, Regina N Tyree, Larry W Raber, Anna N Conner, Jennifer M Pilat, Justin Jacobse, Kara M McNamara, Margaret M Allaman, Gabriella A Raffa, Alain P Gobert, Mohammad Asim, Jeremy A Goettel, Yash A Choksi, Dawn B Beaulieu, Robin L Dalal, Sara N Horst, Baldeep S Pabla, Yuankai Huo, Bennett A Landman, Joseph T Roland, Elizabeth A Scoville, David A Schwartz, M Kay Washington, Yu Shyr, Keith T Wilson, Lori A Coburn, Ken S Lau, Qi Liu
PMID39169060
ABSTRACT

Crohn's disease (CD) is a complex chronic inflammatory disorder with both gastrointestinal and extra-intestinal manifestations associated immune dysregulation. Analyzing 202,359 cells from 170 specimens across 83 patients, we identify a distinct epithelial cell type in both terminal ileum and ascending colon (hereon as 'LND') with high expression of LCN2, NOS2, and DUOX2 and genes related to antimicrobial response and immunoregulation. LND cells, confirmed by in-situ RNA and protein imaging, are rare in non-IBD controls but expand in active CD, and actively interact with immune cells and specifically express IBD/CD susceptibility genes, suggesting a possible function in CD immunopathogenesis. Furthermore, we discover early and late LND subpopulations with different origins and developmental potential. A higher ratio of late-to-early LND cells correlates with better response to anti-TNF treatment. Our findings thus suggest a potential pathogenic role for LND cells in both Crohn's ileitis and colitis.

MATERIALS
Product Number
Brand
Product Description
HPA002695
Sigma-Aldrich
Anti-LCN2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution