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  • Intracellular delivery of functional proteins via decoration with transporter peptides.

Intracellular delivery of functional proteins via decoration with transporter peptides.

Molecular therapy : the journal of the American Society of Gene Therapy (2004-05-04)
Zurab Siprashvili, Jason A Reuter, Paul A Khavari
ABSTRACT

Despite numerous attractive intracellular targets, protein therapeutics have been principally confined to the extracellular space due to the lack of a straightforward way to deliver functional polypeptides to the cell interior. Peptide sequences facilitating intracellular protein delivery have been identified; however, current strategies to apply them require problematic steps, such as generation of new in-frame fusion proteins, covalent chemical conjugation, and denaturation. We have developed a new approach to protein transfer into cells and tissues that relies on single-step decoration by cysteine-flanked, arginine-rich transporter peptides. This approach facilitated cell and tissue delivery of a variety of functional proteins, including antibodies and enzymes. Decoration with transporter peptides thus provides an attractive general means of intracellular delivery of functional proteins in vitro and in tissue.

MATERIALS
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Sigma-Aldrich
Anti-β-Actin–FITC antibody, Mouse monoclonal, clone AC-15, purified from hybridoma cell culture