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  • Innovative particle standards and long-lived imaging for 2D and 3D dSTORM.

Innovative particle standards and long-lived imaging for 2D and 3D dSTORM.

Scientific reports (2019-12-01)
Angelina Provost, Corentin Rousset, Laura Bourdon, Sarra Mezhoud, Emma Reungoat, Camille Fourneaux, Timothée Bresson, Marine Pauly, Nicolas Béard, Laura Possi-Tchouanlong, Boyan Grigorov, Philippe Bouvet, Jean-Jacques Diaz, Christophe Chamot, Eve-Isabelle Pécheur, Catherine Ladavière, Marie-Thérèse Charreyre, Arnaud Favier, Christophe Place, Karine Monier
PMID31784555
ABSTRACT

Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM.