Skip to Content
MilliporeSigma
  • Mitochondria-targeted near-infrared fluorescent probe for the detection of carbon monoxide in vivo.

Mitochondria-targeted near-infrared fluorescent probe for the detection of carbon monoxide in vivo.

Talanta (2018-07-22)
Song-Jiao Li, Dong-Ye Zhou, Yong-Fei Li, Bin Yang, Juan Ou-Yang, Jia Jie, Juan Liu, Chun-Yan Li
ABSTRACT

Carbon monoxide is a critical gasotransmitter in the body and related with mitochondrial respiration. To date, various fluorescent probes for CO have been well proposed, but two main problems remain. One is that most of the probes are not mitochondria-targeting, even if the probes claim to be able to detect CO in living cells. The other is that the probes for CO display excitation and emission within the ultraviolet or visible range, which hinders their applications in vivo. Herein, a hemicyanine-based near-infrared (NIR) fluorescent probe named CyAPC is first synthesized and used to detect mitochondrial CO. The characteristics of probe CyAPC are as follows: (1) The fluorescence emission of the sensing system is at 736 nm belonging to NIR region, which is suitable for bioimaging in vivo. (2) CyAPC, a positively charged molecule, would have a high tendency to localize in mitochondria of cells. (3) The fluorescence change of the probe is attributed to the fact that CO with Pd2+ induced cleavage of the allyl formate group from the probe and CyAPC (fluorescence off) is transformed into CyOH (fluorescence on), which is proved by HPLC, MS and DFT calculation. (4) The NIR fluorescent probe is applied for the detection of exogenous and endogenous CO in various biological samples such as cell, tissue and in vivo with satisfactory results.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,1,2-Trimethylbenz[e]indole, ≥98.0% (HPLC)
Sigma-Aldrich
Iodoethane, contains copper as stabilizer, ReagentPlus®, 99%