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  • Inhibitory effect of quinoa and fenugreek extracts on pancreatic lipase and α-amylase under in vitro traditional conditions or intestinal simulated conditions.

Inhibitory effect of quinoa and fenugreek extracts on pancreatic lipase and α-amylase under in vitro traditional conditions or intestinal simulated conditions.

Food chemistry (2018-09-04)
Teresa Herrera, Joaquín Navarro Del Hierro, Tiziana Fornari, Guillermo Reglero, Diana Martin
ABSTRACT

Ethanol extracts (EE) from fenugreek and quinoa seeds with different total content of inhibitory compounds (TIC, total saponin plus phenolic) were prepared with and without concentration of TIC (CEE -concentrated EE-, and EE, respectively). Their inhibitory activity on pancreatic lipase and α-amylase was assessed by traditional in vitro methods (with or without orbital shaking), and by simulating intestinal digestion. CEE contained higher contents of TIC than EE, being fenugreek superior to quinoa (p < 0.001). The extracts inhibited enzymes in a dose-dependent manner, CEE extracts being stronger (fenugreek for lipase -p = 0.009-, and quinoa for α-amylase -p < 0.001-). Shaking did not impact the activity. Intestinal conditions worsened the inhibition of lipase, but slightly catalyzed the α-amylase. Longer times of reaction worsened activities. The importance of assessing the inhibitory activity of extracts under simulated intestinal conditions is concluded, being fenugreek more interesting than quinoa, especially against pancreatic lipase.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lugol solution, for microscopy (Bact., Bot.)
Sigma-Aldrich
Lipase from porcine pancreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
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Starch from potato, Soluble
Millipore
Bile salts, suitable for microbiology
Sigma-Aldrich
α-Amylase from porcine pancreas, Type VI-B, ≥5 units/mg solid
SAFC
Dulbecco′s Phosphate Buffered Saline, liquid, sterile-filtered, with calcium, with magnesium, suitable for cell culture
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4-Methylumbelliferyl oleate, suitable for fluorescence, ≥95% (HPCE)
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Soyasaponin I
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(+)-Catechin, analytical standard
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Protodioscin, from Dioscorea spp., ≥98% (HPLC)