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  • Separation of mono-, di-, and trihydroxy stereoisomers of bile acids by capillary gas-liquid chromatography.

Separation of mono-, di-, and trihydroxy stereoisomers of bile acids by capillary gas-liquid chromatography.

Journal of lipid research (1983-02-01)
T Iida, F C Chang, T Matsumoto, T Tamura
ABSTRACT

Capillary gas-liquid chromatographic separation was studied for the complete set of the 26 theoretically possible isomers of mono-, di-, and trihydroxylated 5 beta-cholanic acids, which differ from one another in the number, position, and configuration of hydroxyl groups at C-3, C-7, and/or C-12 in the nucleus, as well as for some of their related acids. The bile acid samples were chromatographed as their methyl ester-trimethylsilyl (TMSi) ether derivatives and analyzed on three capillary columns coated with nonpolar OV-1, slightly polar OV-17, and polar SP-2340 as liquid phases. The retention times on capillary gas-liquid chromatography (GLC) responded dramatically to the minor structural differences, and almost complete separation of the positional and stereochemical isomers was achieved by the combined use of SP-2340 and OV-17 (or OV-1) capillary columns.

MATERIALS
Product Number
Brand
Product Description

Supelco
SP®-2340 Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2340 Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2340 Capillary GC Column, L × I.D. 60 m × 0.32 mm, df 0.20 μm