ZRB2167

Anti-SLC12A4 Antibody, clone 1F16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, Solute carrier family 12 member 4, hKCC1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F16, recombinant monoclonal
• description: 1F16 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 120.65 kDa; observed mol wt ~30 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_005063
• UniProt accession no.: Q9UP95
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SLC12A4(6560)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Solute carrier family 12 member 4 (SLC12A4). It targets an epitope within 20 amino acids from the cytoplasmic domain with in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the cytoplasmic domain within the C-terminal region of human Solute carrier family 12 member 4 (SLC12A4).

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SLC12A4 in Human pancreas tissue sections.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound the recombinant fragment of Human SLC12A4 with a KD of 1.4 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A4 in HeLa and K562 cells.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected the recombinant fragment of Human SLC12A4.

Enzyme Immunoassay ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant fragment of Human SLC12A4.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Solute carrier family 12 member 4 (UniProt: Q9UP95; also known as Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, hKCC1) is encoded by the SLC12A4 (also known as KCC1) gene (Gene ID: 6560) in human. Potassium-coupled chloride transporters (KCCs; SLC12A) play crucial role in regulating cell volume and intracellular chloride concentration. SLC12A4 is a homomultimeric, multi-pass membrane protein with seven cytoplasmic domains and twelve transmembrane domains. It can also form heteromultimers with other K-Cl cotransporters. It is ubiquitously distributed with higher expression observed in erythrocytes from patients with hemoglobin (Hb) SC and Hb SS compared to normal AA erythrocytes. This contributes to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of Hbs. SLC12A4 mediate electroneutral potassium-chloride co-transport when activated by cell swelling. It is involved in volume homeostasis in single cells. Seven different isoforms of SLC12A4 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, GP., et al. (2004). Blood. 103(11); 4302-4309; Chi, G., et al. (2021). EMBO J. 40(14); e107294; Mercado, A., et al. (2000). J. Biol. Chem. 275(39); 30326-30334).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable