SAB4200809

Anti-Epidermal Growth Factor (EGF) Receptor, Mouse monoclonal

clone 29.1, purified from hybridoma cell culture

• Synonym(s): EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 29.1, monoclonal
• form: buffered aqueous solution
• mol wt: ~170 kDa
• species reactivity: human, bovine, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.; immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.
• isotype: IgG1
• UniProt accession no.: P00533
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956)

Description

General description

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.

Immunogen

Paraformaldehyde-fixed carcinoma cell line A431

Application

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable