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Key Documents

MAK547

Sigma-Aldrich

High Sensitivity Beta-Hydroxybutyrate (Ketone Body) Assay Kit

sufficient for 200 fluorometric tests

Synonym(s):

High Sensitivity Beta-Hydroxybutyrate Assay Kit

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About This Item

UNSPSC Code:
41116104
NACRES:
NA.21

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases

shipped in

dry ice

storage temp.

−20°C

General description

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop. The two main ketone bodies are beta-hydroxybutyrate (β-HB) and acetoacetate, while acetone is the third abundant ketone body. Normally these two predominant ketone bodies are present in small amounts in the blood during fasting and prolonged exercise. In patients who have diabetes, alcohol or salicylate poisoning, hormone deficiency, childhood hypoglycemia and other acute disease states, large quantities of ketone bodies are found in the blood. The over-production and accumulation of ketone bodies in the blood (ketosis) can lead to pathological metabolic acidosis (ketoacidosis). Blood ketone testing methods that quantify β-HB, the predominant ketone body in the blood (approximately 75%) have been used for diagnosing and monitoring treatment of ketoacidosis.

​The Beta-Hydroxybutyrate Assay Kit offers a sensitive fluorescent assay for measuring Beta-HB levels in biological samples. This assay is based on an enzyme coupled reaction of β-HB, in which the product NADH can be specifically monitored by a fluorescent NADH sensor. The detection limit of this assay kit is 1.4 µM β-HB in a 100 µL reaction volume.

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3


Certificates of Analysis (COA)

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