Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

MAK540

Sigma-Aldrich

Beta-Hydroxybutyrate (Ketone Body) Assay Kit

sufficient for 200 colorimetric tests

Synonym(s):

Beta-Hydroxybutyrate Quantification Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41116104
NACRES:
NA.25

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

shipped in

dry ice

storage temp.

−20°C

General description

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop. The two main ketone bodies are beta-hydroxybutyrate (β-HB) and acetoacetate (AcAc), while acetone is the third abundant ketone body. Normally these two predominant ketone bodies are present in small amounts in the blood during fasting and prolonged exercise. In patients who have diabetes, alcohol or salicylate poisoning, hormone deficiency, childhood hypoglycemia and other acute disease states, large quantities of ketone bodies are found in the blood. The over-production and accumulation of ketone bodies in the blood (ketosis) can lead to pathological metabolic acidosis (ketoacidosis). Blood ketone testing methods that quantify beta-HB, the predominant ketone body in the blood (approximately 75%) have been used for diagnosing and monitoring treatment of ketoacidosis.

Suitability

The Beta-Hydroxybutyrate Assay Kit offers a sensitive colorimetric assay for measuring βHB levels in biological samples.

Principle

This assay is based on an enzyme coupled reaction of beta-HB, in which the product NADH can be specifically monitored by a NADH sensor. The signal can be measured by an absorbance microplate reader with the OD ratio at the wavelength of 570 nm to 610 nm. The detection limit of this assay kit is 4 µM beta-hydroxybutyrate in a 100 µL reaction volume.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service