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MAK463

Sigma-Aldrich

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Hydroxyproline Assay Kit (Perchlorate-free)

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

100

application(s)

cosmetics

detection method

colorimetric

relevant disease(s)

dermatological diseases; rheumatological diseases; neurological disorders; cancer; cardiovascular diseases

shipped in

ambient

storage temp.

2-8°C

General description

Hydroxyproline is a unique modified amino acid that is found exclusively in several animal proteins, the most prevalent of which is collagen. Collagen is a key structural protein of the extracellular matrix and is common in connective tissues due to its incredible strength. Because of its role as a key structural protein in animals, collagen is crucial for many medical applications, such as tissue transplantation and scaffolding for complex cell cultures. Collagen is also used in the beauty industry, as it can be supplemented to increase tissue rigidity, resulting in skin that appears more youthful.

Features and Benefits

  • Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline
  • Assay uses an improved perchlorate-free chemistry

Suitability

The kit is suitable for hydroxyproline and collagen detection in biologic (e.g., serum, cells, urine, and tissue lysate) and cosmetic samples.

Principle

In the first step of this procedure, hydroxyproline in the sample is oxidized to a pyrrole ring. This compound then reacts with a dye reagent to yield a pink product that can be measured at 560 nm.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H315,H317,H318,H334

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Certificates of Analysis (COA)

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