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Key Documents

H4891

SAFC

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate, phenol red and sodium bicarbonate, powder, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Balanced Salt Solution, Hanks’ Salt Solution

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Formulation: click here.

Application

Hanks′ Balanced Salts may be used when working with stem cells or when growing cells at low densities. Also, it may be used to minimize cell attachment or aggregation, and for the preparation of external bathing solution and wash solution.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Tomoko Ishikawa et al.
International journal of molecular medicine, 35(5), 1273-1289 (2015-03-18)
The human placental transfer of maternal IgG is crucial for fetal and newborn immunity. Low-affinity immunoglobulin gamma Fc region receptor IIb2 (FCGR2B2 or FcγRIIb2) is exclusively expressed in an IgG-containing, vesicle-like organelle (the FCGR2B2 compartment) in human placental endothelial cells;
Michael C Kelly et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(19), 6699-6710 (2012-05-11)
Hearing impairment due to the loss of sensory hair cells is permanent in humans. Considerable interest targets the hair cell differentiation factor Atoh1 as a potential tool with which to promote hair cell regeneration. We generated a novel mouse model
Ying Zhang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(18), 2001797-2001797 (2020-10-02)
In the last decade, organoid technology has developed as a primary research tool in basic biological and clinical research. The reliance on poorly defined animal-derived extracellular matrix, however, severely limits its application in regenerative and translational medicine. Here, a well-defined
Y-F Liu et al.
Reproduction (Cambridge, England), 159(4), 493-501 (2020-01-23)
Maintenance of a suitable uterine milieu is important for embryo development and subsequent implantation during early pregnancy. High estrogen level in proestrous and estrous stages is essential for uterine anti-bacterial activity during preimplantation period. Lipocalin-2 is an essential molecule which

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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