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GE25-6600-31

Genomiphi V2 DNA Amplification Kit

Cytiva 25-6600-31, sufficient for 100 reactions

Synonym(s):

DNA Amplification Kit, Genomiphi V2 Kit

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About This Item

UNSPSC Code:
41116006
NACRES:
NA.55

usage

sufficient for 100 reactions

manufacturer/tradename

Cytiva 25-6600-31

shipped in

dry ice

storage temp.

−70°C

General description

Illustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample.

illustra GenomiPhi V2 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for mini-scale whole genome amplification by isothermal strand displacement amplification.

Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.

The illustra GenomiPhi V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 μg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA card lysates, blood and buccal swabs.
GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.

Application

Application For mini-scale whole genome amplification by isothermal strand displacement amplification; typical downstream applications are are genotyping (SNP, STR, array CGH) and DNA archiving.

Features and Benefits

  • Representative isothermal amplification of the whole genome.
  • No template-independent, background amplification product.
  • Outperforms PCR-based whole genome amplification techniques.
  • Quick and simple automation-friendly protocol; no thermal cycler required.
  • Yields high-quality DNA (e.g., for high-throughput genotyping, hybridization, and DNA archival).

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
S-re the kit at -70°C. The enzyme mix must be s-red at -70°C; all other components may be s-red at -20°C Thaw components on ice and maintain at 0°C - 4°C during handling

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
FTA is a trademark of Qiagen Group
GenomiPhi is a trademark of Cytiva
illustra is a trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

GenomiPhi method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.

GenomiPhi method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.

GenomiPhi method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.

GenomiPhi method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service