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Key Documents

Y13-259

89111412, Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

Osborne-Mendel rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-p21 Protein, Harvey Murine Sarcoma Virus

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen

Cell Line Description

The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.

Immunogen

Ha-MuSV transformed syngeneic NRK cells

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask

Other Notes

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