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B8667

Sigma-Aldrich

Bovine Serum Albumin solution

20 mg/mL in H2O, low bioburden, protease free, for molecular biology

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

300

grade

for molecular biology

product line

BioReagent

Assay

20-22 mg/mL protein basis (biuret)

form

solution

quality

non-acetylated

purified by

heat shock fractionation

contains

does not contain preservative

origin

USA origin

concentration

20 mg/mL in H2O
20-22 mg/mL Biuret

technique(s)

PCR: suitable

impurities

Alakaline phosphatase, none detected
 DNase, none detected
 Endonuclease, none detected
 Exonuclease, none detected
 NICKase, none detected
 peroxidase, none detected
<10.0 cfu/mL Total microbial count

UniProt accession no.

P02769

foreign activity

Alkaline Phosphatase free
DNase free
DNase, RNase, NICKase, protease, peroxidase and alkaline phosphatase, none detected
NICKase free
Peroxidase free
Protease free
RNase free

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... ALB(280717)

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Application

Select the right albumin for your application with the Albumin Selection Guide.
BSA has various uses in molecular biology applications, such as:
  • As an added component in solutions of enzymes or of other proteins, to prevent adhesion of the enzyme or other protein of interest to the reaction tube surface or to pipette surfaces
  • As an inert enzyme or protein stabilizer during incubation, to allow the enzyme or protein of interest to function properly

Features and Benefits

This BSA solution product for molecular biology is tested for the following properties:
  • Alkaline Phosphatase-free
  • DNase-free / Exonuclease-free
  • Nickase-free / Endonuclease-free
  • Protease-free
  • RNase-free

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Naveen Ramalingam et al.
Biomicrofluidics, 11(3), 034110-034110 (2017-06-15)
Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates
Elena Beam et al.
Clinical transplantation, 32(1) (2017-11-08)
Cytomegalovirus (CMV) pneumonia causes major morbidity and mortality. Its diagnosis requires demonstration of viral cytopathic changes in tissue, entailing risks of lung biopsy. This study aimed to determine CMV viral load (VL) thresholds in bronchoalveolar lavage fluid (BALF) for diagnosis
Alexander S Day et al.
Scientific reports, 11(1), 19933-19933 (2021-10-09)
An emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results
Oscar Aparicio et al.
Current protocols in cell biology, Chapter 17, Unit 17-Unit 17 (2008-01-30)
Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting the association of individual proteins with specific genomic regions in vivo. Live cells are treated with formaldehyde to generate protein-protein and protein- DNA cross-links between molecules in close
Anjelika Gasilina et al.
Bio-protocol, 12(18) (2022-11-01)
Understanding the molecular and structural mechanisms that govern the assembly and organization of higher-order actin architecture requires the use of in vitro actin binding and bundling assays. Crosslinking of actin filaments into bundles can be monitored in vitro via several
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