48300

Bile esculin agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Esculin Bile Agar
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• Agency: according to ISO 10273:2017
• Quality Level: 200
• sterility: non-sterile
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; esculin, 1 g/L ; ferric citrate, 0.5 g/L ; meat extract, 3 g/L ; meat peptone, 5 g/L ; ox-bile, 40 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable
• final pH: 6.6±0.2 (25 °C)
• application(s): environmental; food and beverages; water monitoring; microbiology
• suitability: Streptococcus spp.; selective and differential for Enterococcus spp.

Description

Application

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).

Preparation Note

Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable