Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

MAP2MAG-76K

Millipore

MILLIPLEX® Mouse Acute Phase Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

The analytes available for this multiplex kit are: Adipsin, Alpha-1 Acid Glycoprotein (AGP), Alpha-2 Macroglobulin (A2M), C-Reactive Protein, Haptoglobin, SAP.

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 99.2-128.2%
sensitivity: 0.001-7.495 ng/mL
standard curve range: 0.001-0.5 ng/mL
(AGP)

standard curve range: 0.005-4.0 ng/mL
(Adipsin)

standard curve range: 0.014-10 ng/mL
(CRP)

standard curve range: 0.549-400 ng/mL
(Haptoglobin)

standard curve range: 13.717-10,000 ng/mL
(SAP)

standard curve range: 6.858-5000 ng/mL
(A2M)

inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Acute phase proteins are a class of proteins whose circulating concentrations increase (positive acute phase proteins) or decrease (negative acute phase proteins) in response to inflammation. This response is called the acute phase reaction or acute phase response. In recent years several new acute phase proteins have been identified, some of which are secreted from adipose tissue.

MILLIPLEX® Mouse Acute Phase Magnetic Bead Panel 2 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum or plasma samples, mouse tissue extract, or cell/tissue culture supernatant samples: Adipsin (Complement Factor D), Alpha-1 Acid Glycoprotein (AGP), C-Reactive Protein (CRP), Haptoglobin (HPTGN), Alpha-2 Macroglobulin (A2M) and Serum Amyloid Protein/Pentraxin-2 (SAP). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Specificity

Cross Reactivty
Antibody pairs in the panel are specific and demonstrate no cross-reactivity (<0.1%) with
other analytes in the panel.

Application

  • Analytes: α-1, Acid Glycoprotein (AGP), α-2 Macroglobulin (A2M), Adipsin (Factor D), C-Reactive Protein (CRP), Haptoglobin, Serum Amyloid P (SAP)
  • Recommended Sample Type: Mouse serum, plasma or cell/tissue culture supernatants lysates
  • Recommended Sample Dilution: 25 μL per well of 1:20,000 diluted serum or plasma; cell/tissue culture samples may require dilution in appropriate control medium
  • Assay Run Time: Overnight (16-22 hours) at 2-8°C
  • Research Category: Cardiovascular
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

R B Kostogrys et al.
The journal of nutrition, health & aging, 19(7), 710-718 (2015-07-22)
Low Carbohydrate High Protein diet represents a popular strategy to achieve weight loss. The aim of this study was to characterize effects of low carbohydrate, high protein diet (LCHP) on atherosclerotic plaque development in brachiocephalic artery (BCA) in apoE/LDLR-/- mice
Edward O List et al.
Molecular endocrinology (Baltimore, Md.), 27(3), 524-535 (2013-01-26)
GH receptor (GHR) gene-disrupted mice (GHR-/-) have provided countless discoveries as to the numerous actions of GH. Many of these discoveries highlight the importance of GH in adipose tissue. For example GHR-/- mice are insulin sensitive yet obese with preferential
Marnix Lameijer et al.
Nature biomedical engineering, 2(5), 279-292 (2019-04-03)
Macrophage accumulation in atherosclerosis is directly linked to the destabilization and rupture of plaque, causing acute atherothrombotic events. Circulating monocytes enter the plaque and differentiate into macrophages, where they are activated by CD4+ T lymphocytes through CD40-CD40 ligand signalling. Here
Damir Nizamutdinov et al.
Scientific reports, 7, 40112-40112 (2017-01-21)
Annually, there are over 2 million incidents of traumatic brain injury (TBI) and treatment options are non-existent. While many TBI studies have focused on the brain, peripheral contributions involving the digestive and immune systems are emerging as factors involved in
Sarah M Snyder et al.
The Journal of nutrition, 146(5), 1001-1007 (2016-04-08)
Intake of polyphenols and polyphenol-rich fruit extracts has been shown to reduce markers of inflammation, diabetes, and hepatic complications that result from the consumption of a high-fat (HF) diet. The objective of this study was to determine whether mice fed

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service