MABF3034
Anti-NKG2D/CD314 Antibody, clone A10
Synonym(s):
B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13B
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
hamster
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A10, monoclonal
species reactivity
mouse
packaging
antibody small pack of 100
technique(s)
flow cytometry: suitable
inhibition assay: suitable
isotype
IgGκ
epitope sequence
Extracellular domain
Protein ID accession no.
UniProt accession no.
Gene Information
mouse ... Tnfsf13b(27007)
Specificity
Clone A10 is an Armenian Hamster monoclonal antibody that detects NKG2-D type II integral membrane protein (NKG2D/CD314).
Immunogen
Recombinant fragment containing the extracellular domain of mouse NKG2D/CD314.
Application
Quality Control Testing
Evaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.
Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected NKG2D/CD314 in one million C57BL/6 mouse splenocytes.
Tested Applications
Flow Cytometry Analysis: A representative lot detected NKG2D/CD314 in Flow Cytometry applications (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).
Agonist Function: A representative lot stimulated the production of GM-CSF by KY-1 NK clones and C57BL/6 LAK cells and also stimulated NK cells to kill Daudi targets. (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).
Agonist Functon: A representative lot increased Vav phosphorylation in freshly isolated splenic C57BL/6 NK cells. (Ghasemi, R., et al. (2016). Nat Commun. 7:12878).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.
Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected NKG2D/CD314 in one million C57BL/6 mouse splenocytes.
Tested Applications
Flow Cytometry Analysis: A representative lot detected NKG2D/CD314 in Flow Cytometry applications (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).
Agonist Function: A representative lot stimulated the production of GM-CSF by KY-1 NK clones and C57BL/6 LAK cells and also stimulated NK cells to kill Daudi targets. (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).
Agonist Functon: A representative lot increased Vav phosphorylation in freshly isolated splenic C57BL/6 NK cells. (Ghasemi, R., et al. (2016). Nat Commun. 7:12878).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).
Physical form
Purified Armenian Hamster monoclonal antibody IgG in PBS without azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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