539578

PKG, Iα, Bovine Lung

• Synonym(s): cGMP-Dependent Protein Kinase Catalytic Subunit, PKG _Iα Catalytic Subunit, Protein Kinase G Iα, cGMP-Dependent Protein Kinase Catalytic Subunit, PKG_Iα Catalytic Subunit, Protein Kinase G Iα
• UNSPSC Code: 12352202
• NACRES: NA.42

Properties

• form: liquid
• Quality Level: 100
• specific activity: ≥1300 units/μg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Note: 4µg = 6000 units.

Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.

Physical form

In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable