444258

MMP-14 Substrate I, Fluorogenic

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

• Synonym(s): MMP-14 Substrate I, Fluorogenic, MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
• Empirical Formula (Hill Notation): C₆₆H₈₂N₁₆O₁₇S
• Molecular Weight: 1403.52
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~328 nm; λ_em ~400 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (k_cat/k_m = 730,000 M^-1s^-1 for MMP-14 (MT1-MMP) and 36,000 M^-1s^-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (k_cat/K_m = 7.3 x 10⁵ M^-1s^-1 for MMP-14 (MT1-MMP) and 3.6 x 10⁴ M^-1s^-1 for MMP-11 at 25°C, pH 7.5).

Biochem/physiol Actions

Cell permeable: no

Primary Target
MMP-14

Product does not compete with ATP.

Reversible: no

kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

MCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Mucha, A., et al. 1998. J. Biol. Chem.273, 2763.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable