1.10455

Columbia agar (base)

for the cultivation of fastidious microorganisms (According harm. EP/USP/JP and ISO)

• UNSPSC Code: 41106213

Properties

• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 7.3 (25 °C, 42 g/L in H₂O)
• solubility: 42 g/L
• bulk density: 646 kg/m³
• application(s): microbiology; pharmaceutical
• storage temp.: 15-25°C
• suitability: Clostridium spp.

Description

General description

for the cultivation of fastidious microorganisms (According harm. EP/USP/JP and ISO)

Analysis Note

Appearance (clearness)clear to slightly opalescentAppearance (color)yellowish-brownpH-value (25 °C)7.1 - 7.5Gel strength (Gelomat assay) (Base)≥ 50 gGel strength (Gelomat assay) (with blood)≥ 40 gTypical Composition (g/litre): Peptone from casein10.0; Peptone from meat 5.0; Heart extract 3.0; Extract from yeast 5.0; Starch 1.0; Sodium chloride 5.0; Agar-Agar 13.0.Growth promotion test in accordance with the harmonised method of EP, USP and JP.Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))10 - 100Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))10 - 100Inoculum on reference medium (Streptococcus pyogenes ATCC 12344)10 - 100Inoculum on reference medium (Streptococcus pyogenes ATCC 21059)10 - 100Inoculum on reference medium (Streptococcus pneumoniae ATCC 6301)10 - 100Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))10 - 100Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001))10 - 100Inoculum on reference medium (Clostridium sporogenes ATCC 11437)10 - 100Inoculum on reference medium (Clostridium sporogenes ATCC 19404 (WDCM 00008))10 - 100Colony count (Escherichia coli ATCC 8739 (WDCM 00012))Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))Colony count (Streptococcus pyogenes ATCC 12344)Colony count (Streptococcus pyogenes ATCC 21059)Colony count (Streptococcus pneumoniae ATCC 6301)Colony count (Enterococcus faecalis ATCC 19433 (WDCM 00009))Colony count (Bacillus cereus ATCC 11778 (WDCM 00001))Colony count (Clostridium sporogenes ATCC 11437)Colony count (Clostridium sporogenes ATCC 19404 (WDCM 00008))Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 70 %Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))≥ 70 %Recovery on test medium (Streptococcus pyogenes ATCC 12344)≥ 70 %Recovery on test medium (Streptococcus pyogenes ATCC 21059)≥ 70 %Recovery on test medium (Streptococcus pneumoniae ATCC 6301)≥ 70 %Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))≥ 70 %

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3