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230162

Sigma-Aldrich

Sulforhodamine B

Dye content 75 %

Synonym(s):

Acid Red 52, Sulforhodamine B monosodium salt

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About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
3520-42-1
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein:
3886008
EC Number:
222-529-8
MDL number:
MFCD00010180
UNSPSC Code:
12171500
PubChem Substance ID:
24853778
NACRES:
NA.47

Assay

≥73% (HPLC)

form

powder

composition

Dye content, 75%

color

brown to very dark brown
 green to very dark green

solubility

methanol: 1 mg/mL

λmax

554 nm

ε (extinction coefficient)

≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sulforhodamine B colorimetric assay for cytotoxicity screening
Vichai V
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