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Key Documents

201464

Sigma-Aldrich

Variamine Blue RT Salt

Synonym(s):

4-Aminodiphenylamine diazonium sulfate, 4-Diazodiphenylamine sulfate, Azoic Diazo Component 22, Azoic Diazo No. 22

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About This Item

Linear Formula:
C6H5NHC6H4N2 · HSO4
CAS Number:
Molecular Weight:
293.30
Colour Index Number:
37240
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

solid

solubility

1 M HCl: 10 mg/mL

λmax

377 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OS([O-])(=O)=O.N#[N+]c1ccc(Nc2ccccc2)cc1

InChI

1S/C12H10N3.H2O4S/c13-15-12-8-6-11(7-9-12)14-10-4-2-1-3-5-10;1-5(2,3)4/h1-9,14H;(H2,1,2,3,4)/q+1;/p-1

InChI key

FMRQRWPEQSPSDG-UHFFFAOYSA-M

Application

Variamine Blue RT Salt has been used as a dye in ultraviolet-visible spectrophotometric method based-uricase enzyme for the detection of uric acid in normal urine. It is also used in combination with naphthol AS-MX phosphate-variam to determine the cytochemical visualization of alkaline phosphatase activity.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Localization Mechanisms in Enzyme Cytochemistry
Studied with Alkaline Phosphatase-loaded
Erythrocyte Ghosts?
RAAP A K
The Journal of Histochemistry and Cytochemistry, 32, 815-820 (1984)

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