Skip to Content
MilliporeSigma
All Photos(3)

Documents

198250

Sigma-Aldrich

Rose bengal

certified by the Biological Stain Commission, Dye content ≥80 %

Synonym(s):

4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C20H2Cl4I4Na2O5
CAS Number:
632-69-9
Molecular Weight:
1017.64
Colour Index Number:
45440
Beilstein:
3645857
EC Number:
211-183-3
MDL number:
MFCD00005043
UNSPSC Code:
12171500
PubChem Substance ID:
24851870
NACRES:
NA.47

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥80%

color

dark violet

solubility

H2O: 1 mg/mL

λmax

548 nm

ε (extinction coefficient)

≥90000 at 546-550 nm at 0.008 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24

InChI

1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2

InChI key

UWBXIFCTIZXXLS-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C20H2O5I4Cl4Na2. It is an anionic water-soluble xanthene dye.

Application

Rose bengal has several microbilogical staining applications including:
  • Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
  • counterstaining hematoxylin
  • staining cellular inclusions, together with Bismark brown



Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Steffanie Heindl et al.
The Journal of experimental medicine, 218(8) (2021-05-27)
Neuroinflammation is an emerging focus of translational stroke research. Preclinical studies have demonstrated a critical role for brain-invading lymphocytes in post-stroke pathophysiology. Reducing cerebral lymphocyte invasion by anti-CD49d antibodies consistently improves outcome in the acute phase after experimental stroke models.
Priyanka S Bapat et al.
Frontiers in cellular and infection microbiology, 11, 713092-713092 (2021-09-21)
Fungal infections are increasing in prevalence worldwide. The paucity of available antifungal drug classes, combined with the increased occurrence of multidrug resistance in fungi, has led to new clinical challenges in the treatment of fungal infections. Candida auris is a
J Varga et al.
Studies in mycology, 69(1), 1-17 (2011-09-06)
Four new species, Aspergillus eucalypticola, A. neoniger, A. fijiensis and A. indologenus are described and illustrated. Aspergillus eucalypticola was isolated from Eucalyptus leaf from Australia, and is related to A. tubingensis and A. costaricaensis, but could clearly be distinguished from
Rebecca Sadler et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(5), 1162-1173 (2020-01-01)
Recovery after stroke is a multicellular process encompassing neurons, resident immune cells, and brain-invading cells. Stroke alters the gut microbiome, which in turn has considerable impact on stroke outcome. However, the mechanisms underlying gut-brain interaction and implications for long-term recovery
Priyanka Bapat et al.
Microorganisms, 9(3) (2021-03-04)
Fungal infections are increasing in prevalence worldwide, especially in immunocompromised individuals. Given the emergence of drug-resistant fungi and the fact that there are only three major classes of antifungal drugs available to treat invasive fungal infections, there is a need
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service