Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

357634

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

268259

Sigma-Aldrich

Nickel, foil, thickness 0.125 mm, ≥99.9%

221678

Sigma-Aldrich

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510041

Sigma-Aldrich

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203904

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

510068

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 3202, slurry, in H₂O, active catalyst

510033

Sigma-Aldrich

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267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

268283

Sigma-Aldrich

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267058

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

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Nickel, sphere, 100pcs, diameter 1.0mm, precision sphere grade 200, 99%

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Nickel, insulated wire, 10m, conductor diameter 0.025mm, insulation thickness 0.0035mm, polyimide insulation

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GF14281815

Nickel, foil, 25x25mm, thickness 2.0mm, as rolled, 99.99+%

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Nickel, tube, 500mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, hard, 99.5%

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Nickel, foil, 25x25mm, thickness 0.15mm, annealed, 99%

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Nickel, wire reel, 10m, diameter 0.75mm, as drawn, 99%

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Nickel, foil, 150x150mm, thickness 0.3mm, annealed, 99%

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Nickel, wire reel, 100m, diameter 0.75mm, as drawn, 99%

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Nickel, foil, 0.5m coil, thickness 0.01mm, 99.95%

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Nickel, foil, 50x50mm, thickness 0.1mm, annealed, 99%

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Nickel, foil, 25x25mm, thickness 0.50mm, annealed, 99%

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Nickel, pellets, 20g, max. size 10mm, 99.99+%

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Nickel, rod, 100mm, diameter 1.5mm, 99.99+%

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Nickel, mesh, 65x65mm, nominal aperture 0.04mm, thickness 0.004mm, wire diameter 0.011mm, 500 wires/inch, open area 60%, electro-formed mesh, 99.9%

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Nickel, wire reel, 1m, diameter 0.75mm, as drawn, 99.99+%

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Nickel, wire reel, 1m, diameter 0.01mm, as drawn, 99.98%