DNA duplex stabilization in crowded polyanion solutions.

DNA duplex stabilization in crowded polyanion solutions.

Chemical communications (Cambridge, England) (2013-01-11)

Imran Khimji, Jeehae Shin, Juewen Liu

ABSTRACT

The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.

MATERIALS

Product Number

Brand

Product Description

181293

Supelco

Poly(acrylic acid), analytical standard, average M_n 130,000 (Typical)

323667

Sigma-Aldrich

Poly(acrylic acid)

306215

Sigma-Aldrich

Poly(acrylic acid), average M_v ~1,250,000

81123

Supelco

Poly(acrylic acid sodium salt), analytical standard, for GPC, 16,000

306223

Sigma-Aldrich

Poly(acrylic acid), average M_v ~3,000,000

420344

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~2,100

181285

Sigma-Aldrich

Poly(acrylic acid), average M_v ~450,000

447013

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~5,100 by GPC, powder

306231

Sigma-Aldrich

Poly(acrylic acid), average M_v ~4,000,000

535931

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~2,000, 50 wt. % in H₂O, electronic grade

416029

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~8,000, 45 wt. % in H₂O

523925

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~100,000, 35 wt. % in H₂O

416010

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~1,200, 45 wt. % in H₂O

416002

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~250,000, 35 wt. % in H₂O