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  • Zn(II) and Cd(II)-based complexes for probing the enzymatic hydrolysis of Na4P2O7 by alkaline phosphatase in physiological conditions.

Zn(II) and Cd(II)-based complexes for probing the enzymatic hydrolysis of Na4P2O7 by alkaline phosphatase in physiological conditions.

Chemical communications (Cambridge, England) (2011-06-21)
Priyadip Das, Sourish Bhattacharya, Sandhya Mishra, Amitava Das
ABSTRACT

Newly synthesized Zn(II) and Cd(II)-based complexes show unique selectivity towards inorganic pyrophosphate (PPi) in 100% aqueous medium at pH = 7.4 and act as a "turn-on" and "turn-off" real-time assay, respectively, for the evaluation of the enzymatic activity of alkaline phosphatase.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium pyrophosphate tetrabasic, ≥95%
Sigma-Aldrich
Sodium pyrophosphate tetrabasic decahydrate, BioXtra, 99.0-103.0%
Sigma-Aldrich
Sodium pyrophosphate tetrabasic decahydrate, BioUltra, ≥99.5% (T)
Sigma-Aldrich
Sodium pyrophosphate decahydrate, ACS reagent, ≥99%
Sigma-Aldrich
Sodium pyrophosphate dibasic, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Sodium pyrophosphate dibasic, practical grade