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  • Mineral content and microbiological examination of some white cheese in Jeddah, Saudi Arabia during summer 2008.

Mineral content and microbiological examination of some white cheese in Jeddah, Saudi Arabia during summer 2008.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2010-05-18)
Magda M Aly, Madeha N Al-Seeni, Safaa Y Qusti, Nagwa M El-Sawi
ABSTRACT

Different local and exported white cheese samples were collected from different markets in Jeddah during September 2008. Trace and heavy metals including Pb, Zn, Mn, Cu, Fe and Cd were analyzed using atomic absorption spectrometry. The concentration of the tested metals was in the range, Fe(++)>Zn(+++)>Mn(++)>Pb(++)>Cu(++)>Cd(++). The mean concentration of 7.63, 7.19, 0.5, 0.47, 0.16 and 0.14 μg/g was recorded for Fe, Zn, Mn, Pb, Cu and Cd, respectively. The concentration of iron ranged from 3.5 to 11.9 μg/g, zinc from 3.4 to 10.5, manganese from 0.12 to 1.0, lead from 0.14 to 1.14, and copper from 0.09 to 0.22. Yeasts and fungi were counted on Sabouraud and Potato Dextrose media and incubation was carried out at 25°C for 7 and 5 days, respectively. Yeast count and fungi count of cheese were ranged from 0.1 to 0.44CFU/g and from 0.123 to 1.11 CFU/g, respectively. Three out of 20 samples of cheese were contaminated with toxigenic fungi with 5% contamination level. Aflatoxin G1 was recorded in three samples using immunoadsorbent column chromatography with a range from 7 to 13 ppm.

MATERIALS
Product Number
Brand
Product Description

Supelco
Aflatoxin G1 solution, 2 μg/mL in acetonitrile, analytical standard
Aflatoxin G1 solution, 3.78 μg/g in acetonitrile, ERM®, certified reference material
Sigma-Aldrich
Aflatoxin G1, from Aspergillus flavus
Supelco
Aflatoxin G1