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GE28-9365-51

Hisprep™ Fast Flow 16/10

Cytiva 28-9365-51

Synonym(s):

16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9365-51

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

Related Categories

General description

HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Features and Benefits

  • Optimized for convenient scale-up purification of histidine-tagged proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible leakage of Ni2+.
  • High binding capacity, approx. 40 mg/mL medium.
  • Convenient and time-saving 20 mL prepacked HiPrep™ format.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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