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D8899

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym(s):

DNA single stranded

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About This Item

CAS Number:
91080-16-9
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
MFCD00163436
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
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biological source

bovine (calf) thymus

Quality Segment

200

grade

Molecular Biology

form

lyophilized powder

mol wt

~50 kb

composition

ssDNA, ≥65%

storage temp.

−20°C

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay[1] and in DNA-binding assay.[2]

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 25 A260 units.
Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

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Deoxyribonucleic acid, single stranded from calf thymus lyophilized powder

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Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

biological source

bovine (calf) thymus

biological source

bovine pancreas

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

~50 kb

mol wt

-

mol wt

>20 kb

mol wt

-

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Questions

  1. Does the product reanneal after storage and cooling at room temperature? Should we boil the ssDNA prior to use? How many base pairs are included in this ssDNA?

    1 answer

    1. Unfortunately, information regarding the reanneal after storing and cooling at room temperature has not been determined.

      Product D8899 is listed as having a length of approximately 50 kb. For single stranded DNA, the molecular weight of one kb is approximately equal to 3.3 × 105 daltons, so 50 kb has a weight of approximately 16.5 × 106 daltons.

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