HPLC Analysis of Testosterone and 1-Testosterone on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)

mobile phase

[A] water; [B] methanol; (20:80, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 240 nm

injection

5 μL

sample

50 μg/mL in 10:90 water:methanol