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Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis.

Bioresource technology (2010-12-15)
Dharmendra S Dheeman, Gary T M Henehan, Jesus M Frías
RESUMEN

An extracellular thermostable lipase from Amycolatopsis mediterranei DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in a 398-fold purification with 36% final recovery. The purified A. mediterranei DSM 43304 lipase (AML) has an apparent molecular mass of 33 kDa. The N-terminal sequence, AANPYERGPDPTTASIEATR, showed highest similarity to a lipase from Streptomyces exfoliatus. The values of K(m)(app) and V(max)(app) for p-nitrophenyl palmitate (p-NPP) at the optimal temperature (60°C) and pH (8.0) were 0.099±0.010 mM and 2.53±0.06 mmol/min mg, respectively. The purified AML displayed significant activity towards a range of short and long chain triglyceride substrates and p-nitrophenyl esters. Hydrolysis of glycerol ester bonds occurred non-specifically. The purified AML displayed significant stability in the presence of organic solvents (40%, v/v) and catalyzed the synthesis of the flavour ester isoamyl acetate in free and immobilized states.

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Isoamyl acetate, natural, ≥97%, FCC, FG
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Isoamyl acetate, ≥95%, FCC, FG
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Isopentyl acetate, mixture of isomers, reagent grade, 98%
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Isopentyl acetate, anhydrous, ≥99%
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Supelco
Isopentyl acetate, analytical standard
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Isopentyl acetate, SAJ special grade, ≥98.0%
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