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Express method for detection of Amanita phalloides amanitine toxins.

Bulletin of experimental biology and medicine (2006-08-26)
D N Vlaskin, E T Gainullina, O V Klyuster, I V Rybal'chenko, S B Ryzhikov, V F Taranchenko
RESUMEN

Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.

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Sigma-Aldrich
β-Amanitin from Amanita phalloides, ≥85% (HPLC)