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Merck

Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli.

Journal of clinical microbiology (1984-02-01)
R W Trepeta, S C Edberg
RESUMEN

Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and lactose fermentation is read directly from MacConkey agar. It was found that 4-methylumbelliferyl-beta-D-glucuronide could be incorporated directly into a modified MacConkey agar to directly detect the presence of beta-glucuronidase. Other characteristics of MacConkey agar were not affected. The incorporation of 4-methylumbelliferyl-beta-D-glucuronide into modified agar obviated the need for manufacture, quality control, and incubation of reagent-containing test tubes. The time needed to identify E. coli strains was reduced from 1 h to 5 min, and the ability to detect this species in mixed specimens was also enhanced.

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4-Nitrophenyl β-D-glucuronide, ≥98% (TLC)
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4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)
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Millipore
Kovac′s reagent for indoles, suitable for microbiology
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