- Determination of the Predatory Capability of Bdellovibrio bacteriovorus HD100.
Determination of the Predatory Capability of Bdellovibrio bacteriovorus HD100.
Bio-protocol (2017-03-20)
Cristina Herencias, Auxiliadora M Prieto, Virginia Martínez
PMID34458487
RESUMEN
Bdellovibrio bacteriovorus HD100 is an obligate predator that preys upon a wide variety of Gram negative bacteria. The biphasic growth cycle of Bdellovibrio includes a free-swimming attack phase and an intraperiplasmic growth phase, where the predator replicates its DNA and grows using the prey as a source of nutrients, finally dividing into individual cells (Sockett, 2009). Due to its obligatory predatory lifestyle, manipulation of Bdellovibrio requires two-member culturing techniques using selected prey microorganisms ( Lambert et al., 2003 ). In this protocol, we describe a detailed workflow to grow and quantify B. bacteriovorus HD100 and its predatory ability, to easily carry out these laborious and time-consuming techniques.
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Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad