Saltar al contenido
Merck

A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila.

Genome biology (2020-11-19)
Nhan Huynh, Noah Depner, Raegan Larson, Kirst King-Jones
RESUMEN

Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify enzymes with the best overall performance and show that Cas13 could target endogenous Drosophila transcripts in vivo with high efficiency and specificity. We also develop Cas13 applications to edit mRNAs and target mitochondrial transcripts. Our vector collection represents a versatile tool collection to manipulate gene expression at the post-transcriptional level.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Penicilina- Estreptomicina, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Corning® Costar® TC-Placas multipocillo tratadas, size 6 wells, clear, polystyrene plate, flat bottom, case of 50 (individually wrapped), sterile, lid
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
Ammonium iron(III) citrate, reagent grade, powder
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
Ammonium citrate tribasic, ≥97% (titration)
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Sigma-Aldrich
Triton X-100, BioXtra
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad