- Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.
Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.
Clinical chemistry (1978-07-01)
G R Gotelli, R E Stanfill, P M Kabra, F A Farina, L J Marton
PMID77745
RESUMEN
We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.
MATERIALES
Referencia del producto
Marca
Descripción del producto
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad