- Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.
Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.
Journal of biotechnology (2011-12-06)
Kristen L Koterba, Trissa Borgschulte, Michael W Laird
PMID22138638
RESUMEN
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in a cell-density dependent manner by knocking down expression of TXN1 via lentivirus transduction of short hairpin RNA.
MATERIALES
Referencia del producto
Marca
Descripción del producto
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad