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85639

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Sinónimos:

Saline sodium citrate buffer dry blend

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

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grade

for molecular biology

Quality Level

product line

BioReagent

form

powder blend

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

solubility

H2O: 0.263 g/mL, clear, colorless

storage temp.

room temp

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Este artículo
S0902S814093017
SSC Buffer for Northern and Southern blotting, powder blend

85639

SSC Buffer

SSC Buffer for Northern and Southern blotting, powder blend

S0902

SSC Buffer

SSPE Buffer for Northern and Southern blotting, powder blend

S8140

SSPE Buffer

SSC Buffer 20x for Northern and Southern blotting, solution

93017

SSC Buffer 20x

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

BioReagent

form

powder blend

form

powder blend

form

powder blend

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

solubility

H2O: 0.263 g/mL, clear, colorless

solubility

water: 263 mg/mL, clear, colorless

solubility

water, high purity: 20 ×, clear, colorless

solubility

-

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Components

  • Convenient pack sizes
  • Multi-purpose use in hybridizations
  • Easy-to-use powder makes fast, inexpensive solutions
SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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