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Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Acetone, suitable for HPLC

Sigma-Aldrich

Acetone, for residue analysis, JIS 5000

Sigma-Aldrich

Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)

Sigma-Aldrich

Methanol, SAJ special grade

Sigma-Aldrich

Acetone, ≥99.5%, for residue analysis

Sigma-Aldrich

Acetone, for residue analysis, ≥99.5%

Sigma-Aldrich

Acetone, SAJ first grade, ≥99.0%

Sigma-Aldrich

Acetone, JIS special grade, ≥99.5%

Sigma-Aldrich

Hydrochloric acid solution, 0.5 M

Sigma-Aldrich

Hydrochloric acid solution, 12 M

Sigma-Aldrich

Hydrochloric acid solution, 2 M

Sigma-Aldrich

Hydrochloric acid solution, 1 M

Sigma-Aldrich

Hydrochloric acid solution, 6 M

Sigma-Aldrich

Hydrogen chloride – ethanol solution, 0.1 M in ethanol

Sigma-Aldrich

Hydrochloric acid solution, 0.2 M

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

Sigma-Aldrich

Acetone, for chromatography, ≥99.8%

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, 99.93%

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥99% (HPLC), from starch

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Aluminum oxide, mesoporous, MSU-X (wormhole), average pore size 3.8 nm

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥99%

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

Sigma-Aldrich

D-(+)-Trehalose dihydrate, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%